Antithrombin Ⅲ
This product is used on NEPHSTAR® protein analysis system for quantitative determination of human Antithrombin Ⅲ(AT-Ⅲ, AT3) in plasma
Antithrombin Ⅲ is a single-chain glycoprotein of molecular weight 58,000 produced by the liver and consists of 432 amino acids. The reactivity of AT-Ⅲ is regulated by combination with its activator heparin. It is an inhibitor of thrombin, Factor Ⅹa, Factor Ⅶa, Factor Ⅸa, Factor Ⅺa, Factor Ⅻa. There is more AT3 in blood than prothrombin. Blood is able to clot only because the reaction of the inhibitor with thrombin is much slower than the action of thrombin on fibrinogen. Antithrombin Ⅲ is mainly involved in the function of anticoagulant system. Decreased levels of AT-Ⅲ are found in nephrotic syndrome, DIC, deep vein thrombosis, hypercoagulability syndrome, oral contraceptives, pulmonary embolism, direct hepatotoxicity, extra-corporeal circulation and L-asparginase administration. Increased levels of AT3 would not cause pathological changes in general.
Immunonephelometry is applied. This method involves measuring the light scattered by insoluble complexes formed by reaction between specific protein in samples and its respective antiserum, and the amount of scattered light is directly proportional to the concentration of the protein under condition that antiserum is in excess. Concentrations are automatically calculated by reference to a calibration curve stored in the instrument.
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