Anti Thyroid Peroxidase
The device TPO.CE is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against Thyroid Peroxidase in human plasma and sera.
Autoimmunity is the failure of an organism to recognize its own constituent parts as self, which allows an immune response against its own cells and tissues. Any disease that results from such an aberrant immune response is termed an Autoimmune Disease.
Thyroid autoantibodies are frequently found in patients with autoimmune thyroid disease. The two most common of these autoantibodies are antibodies to Thyroglobulin and Thyroid peroxidase.
Antibodies to Thyroglobulin and Thyroid Peroxidase will be found in cases of Hashimoto’s disease, myxedema and Grave’s Disease.
Microplates are coated with a recombinant human thyroid peroxidase antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti TPO IgG are captured, if present, by the solid phase.
After washing out all the other components of the sample, in the 2nd incubation bound anti TPO IgG are detected by the addition of anti hIgG antibody, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti TPO IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated on NIBSC reference standard preparation for anti TPO antibodies (code 66/387).
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