Anti Thyroglobulin IgG
The device TG.CE is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against Thyroglobulin in human plasma and sera.
Autoimmunity is the failure of an organism to recognize its own constituent parts as self, which allows an immune response against its own cells and tissues. Any disease that results from such an aberrant immune response is termed an Autoimmune Disease.
Thyroid autoantibodies are frequently found in patients with autoimmune thyroid disease. The two most common of these autoantibodies are antibodies to Thyroglobulin and Thyroid peroxidase.
Antibodies to Thyroglobulin and Thyroid Peroxidase will be found in cases of Hashimoto’s disease, myxedema and Grave’s Disease.
Microplates are coated with a recombinant human thyroglobulin antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti TG IgG are captured, if present, by the solid phase.
After washing out all the other components of the sample, in the 2nd incubation bound anti TG IgG are detected by the addition of anti hIgG antibody, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti TG IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated on WHO standard preparation for anti TG antibodies (code 65/093).
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