Anti-Streptolysin O
This product is used on NEPHSTAR® protein analysis system for quantitative determination of human Anti-Streptolysin O (ASO) in serum
The group A β -haemolytic streptococci produces various toxins that can act as antigens, one of these exotoxins is streptolysin O. The affected organism produces specific antibodies against streptolysin O. The concentration of ASL (O) in the patient’s serum will enable to establish the degree of infection due to β-haemolytic streptococci. Measuring the ASO antibodies are useful for the diagnostic of rheumatoid fever, acute glomerulonephritis and streptococcal infections. Rheumatic fever is an inflammatory disease affecting connective tissue from several parts of human body as skin, heart, joints etc… and acute glomerulonephritis is a renal infection that affects mainly to renal glomerulus.
Particle-enhanced immunonephelometry is applied. This method involves measuring the light scattered by insoluble complexes formed by reaction between specific protein in samples and its respective antibody covalently coupled to latex particles, and the amount of scattered light is directly proportional to the concentration of the protein under condition that antibody is in excess. The latex particles increase the size of complexes formed and thus the amount of light as well as the test sensitivity. Concentrations are automatically calculated by reference to a calibration curve stored in the instrument.
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2. Klein, G. C., Baker, C. N., Jones, W. L., 21, 999-1001 (1971)