Anti-OVA-IgG1 Mouse ELISA
IgG is the most abundant immunoglobulin and is the main antibody in the secondary immune response. IgG1, with the heavy chain 1 and molecular weight of 146kDa, occupies about 2/3 of total IgG. By limiting antigen to OVA (ovalbumin), and by measuring only IgG1, mouse immune response is simplified for elucidation. Shibayagi’s OVA-IgG1 ELISA Kit is useful for specific measurement of mouse anti-OVA IgG1 antibody.
Research topic
Animal studies
Type
Direct ELISA, Biotin-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate
Sample Requirements
5 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
1.88–120 U/ml
Limit of Detection
0.094 U/ml
Intra-assay (Within-Run)
n = 5; CV = 5%
Inter-assay (Run-to-Run)
n = 3; CV = 5%
– Akiyama K, Karaki M, Kobayshi R, Dobashi H, Ishida T, Mori N. IL-16 variability and modulation by antiallergic drugs in a murine experimental allergic rhinitis model. Int Arch Allergy Immunol. 2009;149 (4):315-2
– Fukui N, Honda K, Ito E, Ishikawa K. Peroxisome proliferator-activated receptor gamma negatively regulates allergic rhinitis in mice. Allergol Int. 2009 Jun;58 (2):247-53
– He M, Ichinose T, Yoshida S, Nishikawa M, Mori I, Yanagisawa R, Takano H, Inoue K, Sun G, Shibamoto T. Urban particulate matter in Beijing, China, enhances allergen-induced murine lung eosinophilia. Inhal Toxicol. 2010 Aug;22 (9):709-18
– Ichinose T, Yoshida S, Hiyoshi K, Sadakane K, Takano H, Nishikawa M, Mori I, Yanagisawa R, Kawazato H, Yasuda A, Shibamoto T. The effects of microbial materials adhered to Asian sand dust on allergic lung inflammation. Arch Environ Contam Toxicol. 2008 Oct;55 (3):348-57
– Ichinose T, Yoshida S, Sadakane K, Takano H, Yanagisawa R, Inoue K, Nishikawa M, Mori I, Kawazato H, Yasuda A, Shibamoto T. Effects of asian sand dust, Arizona sand dust, amorphous silica and aluminum oxide on allergic inflammation in the murine lung. Inhal Toxicol. 2008 May;20 (7):685-94
– Matsumoto K, Inoue H, Fukuyama S, Kan-O K, Eguchi-Tsuda M, Matsumoto T, Moriwaki A, Nakano T, Nakanishi Y. Frequency of Foxp3+CD4CD25+ T cells is associated with the phenotypes of allergic asthma. Respirology. 2009 Mar;14 (2):187-94
– Saito K, Torii M, Ma N, Tsuchiya T, Wang L, Hori T, Nagakubo D, Nitta N, Kanegasaki S, Hieshima K, Yoshie O, Gabazza EC, Katayama N, Shiku H, Kuribayashi K, Kato T. Differential regulatory function of resting and preactivated allergen-specific CD4+ CD25+ regulatory T cells in Th2-type airway inflammation. J Immunol. 2008 Nov 15;181 (10):6889-97
– Saito K, Torii M, Ma N, Tsuchiya T, Wang L, Hori T, Nagakubo D, Nitta N, Kanegasaki S, Hieshima K, Yoshie O, Gabazza EC, Katayama N, Shiku H, Kuribayashi K, Kato T. Differential regulatory function of resting and preactivated allergen-specific CD4+ CD25+ regulatory T cells in Th2-type airway inflammation. J Immunol. 2008 Nov 15;181 (10):6889-97
– Yanase Y, Hiragun T, Uchida K, Ishii K, Oomizu S, Suzuki H, Mihara S, Iwamoto K, Matsuo H, Onishi N, Kameyoshi Y, Hide M. Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization. Biochem Biophys Res Commun. 2009 Sep 25;387 (3):435-9