Anti-Deaminated Gliadin Peptide IgG

The Anti-Deamidated Gliadin Peptide (DGP) IgG test is based on the binding of present antibodies in calibrators, controls or prediluted samples on the synthetic deamidated gliadin peptides (DGP) coated on the inner surface of the wells. After a 30 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components.

An anti-human-IgG horseradish peroxidase conjugate solution recognizes IgG class antibodies bound to the immobilized antigens. After a 30 minutes incubation any excess enzyme conjugate, which is not specifically bound is washed away with wash buffer.

A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solutions color changes into yellow. The amount of color is directly proportional to the concentration of IgG antibodies present in the original sample.