Alpha-1-Antitrypsin in Stool Human ELISA
Alpha-1-antitrypsin (α-1-AT) is a 52 kDa glycoprotein, which is produced by the liver, intestinal macrophages, monocytes and mucous membrane cells of the gut. It belongs to the group of acute phase proteins and is one of the most important proteinase inhibitor. α-1-AT inhibits, beside others, the proteinases trypsin and the elastase of neutrophiles. A lack of α-1-AT leads to an enhanced proteolysis. Only a very small amount of α-1-AT is cleaved or resorbed in the gut. Therefore the measurement of α-1-AT in stool reflects the permeability of the gut during inflammatory processes.
Research topic
Immune Response, Infection and Inflammation
Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Plasma-EDTA, Stool
Sample Requirements
100 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Limit of Detection
1.5 ng/ml
Spiking Recovery
18.75 ng/ml
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