Aldosterone ELISA
Aldosterone is a potent mineral corticoid whose synthesis and release are controlled by the renin-angiotensin system of the body. Aldosterone promotes the reabsorption of sodium in the distal tubules of the kidney resulting in potassium secretion along with sodium retention, which controls the circulating blood volume. Chronic overproduction and secretion of aldosterone leads to hypertension. Measurement of aldosterone levels in serum in conjunction with plasma renin levels can be used to differentiate between primary and secondary aldosteronism.
Type
Competitive ELISA, Immobilized antigen
Applications
Serum, Urine, Plasma
Sample Requirements
50 μl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see the label on the box).
Calibration Curve
Calibration Range
15–1000 pg/ml
Limit of Detection
10 pg/ml
Intra-assay (Within-Run)
CV = 6.5%
Inter-assay (Run-to-Run)
CV = 8.7%
Spiking Recovery
106.5 %
Dilutation Linearity
97.1 %
– Toxqui L, Vaquero MP. Aldosterone changes after consumption of a sodium-bicarbonated mineral water in humans. A four-way randomized controlled trial. J Physiol Biochem. 2016 Jun 29;
– Toxqui L, Vaquero MP. An Intervention with Mineral Water Decreases Cardiometabolic Risk Biomarkers. A Crossover, Randomised, Controlled Trial with Two Mineral Waters in Moderately Hypercholesterolaemic Adults. Nutrients. 2016;8 (7)