ADMA ELISA
The vascular endothelium plays a central role in the regulation of vascular structure and function, mainly due to the formation of endothelium-derived nitric oxide (NO). NO is formed by the enzyme NO synthetase (NOS) from the amino acid precursor L-arginine. NOS activity can be downregulated by ADMA, an endogenous inhibitor of NOS. Elevated ADMA levels in plasma have been found in clinical studies including patients with hypercholesterolemia, hypertension, chronic heart failure, chronic renal failure and other internal disorders. ADMA may have diagnostic relevance as a novel cardiovascular risk marker.
Research topic
Blood pressure regulation and NO metabolism, Cardiovascular disease
Type
Competitive ELISA, Immobilized antigen
Applications
Serum, Plasma-EDTA
Sample Requirements
20 μl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see the label on the box).
Calibration Curve
Calibration Range
0.1–5.0 µmol/l
Limit of Detection
0.05 μmol/l
Intra-assay (Within-Run)
CV = 6.1%
Inter-assay (Run-to-Run)
CV = 9.5%
Spiking Recovery
97,00%
Dilutation Linearity
97,00%
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