1. Home
  2. Clinical Chemistry
  3. Liver Markers
  4. Adenosine Deaminase (ADA)

Adenosine Deaminase (ADA)

Adenosine deaminase (ADA) assay kit is for determination of ADA activity in serum and plasma samples.

Regulatery Status: FDA 510(K), CE

Product Catalog Number Packaging Method/Format
Product Catalog No: Kit (250 Tests) Pack Size: DZ117A-K Pack Size: R1: 1 x 50 mL
R2: 1 x 25 mL
Calibrator Included
(Packaged Separately) 		Pack Size: Colorimetric/Kinetic
Liquid Stable
Product Catalog No: Calibrator Pack Size: DZ117A-Cal Pack Size: 1 x 1 mL
Pack Size:
Product Catalog No: Control Pack Size: DZ117A-Con Pack Size: 2 x 2 mL
Pack Size:

Category:
Product Features

The assay is linear from 0-200 U/L and has precisions of Intra assay %CV < 4.5% and Inter assay %CV < 5.0%.The assay is ready-to-use for both manual method and automated chemistry analyzers (Kinetic). The assay is specific for ADA and has no detectable reaction with other nucleosides and is not affected by serum bilirubin up to 30 mg/dL, hemoglobin up to 200 mg/dL, triglycerides up to 750 mg/dL, or ascorbic acid up to 4 mg/dL. The assay is based on H2O2 detection, which is more sensitive and reliable than the method detecting ammonia production.

Assay Principle

Diazyme’s ADA Assay is based on the enzymatic deamination of adenosine to inosine which is converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase (XOD). H2O2 is further reacted with N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT) and 4-aminoantipyrine (4-AA) in the presence of peroxidase (POD) to generate quinone dye which is monitored in a kinetic manner. The entire enzymatic reaction scheme is shown below.

Order Enquiry

    Order Enquiry Form

    References
    1. Feres, M. C., M. C. Martino, S. Maldjian, F. Batista, A. Gabriel, and S. Tufik. “Automatized Adenosine Deaminase Activity Determination in Pleural Fluid and Cerebral Spinal Fluid.” OASIS – Online Abstract Submission and Invitation System. Coe-Truman Technologies, Inc., 24 July 2008. Web. 13 June 2016.
    2. Delacour, Hervé, Christophe Sauvanet, Franck Ceppa, and Pascal Burnat. “Analytical Performances of the Diazyme ADA Assay on the Cobas® 6000 System.” Clinical Biochemistry 43.18 (2010): 1468-471. Web.
    3. Song, David; Lun, Andrea R and Chiu, Weldon. Diazyme Adenosine Deaminase in the Diagnosis of Tuberculous Pleural Effusion: Method Evaluation and Clinical Experiences in a New Zealand Population [online]. New Zealand Journal of Medical Laboratory Science, Vol. 64, No. 1, Apr 2010: 11-13.
    4. Morisson, Patrizio, & Neves, Denise Duprat. (2008). Evaluation of adenosine deaminase in the diagnosis of pleural tuberculosis: a Brazilian meta-analysis. Jornal Brasileiro de Pneumologia, 34(4), 217- 224. https://dx.doi.org/10.1590/S1806-37132008000400006
    5. Delacour, Hervé, Aurore Bousquet, Eléonore Fontan, and Franck Ceppa. “Ammonia Does Not Interfere with the Diazyme Adenosine Deaminase Test.” Clinical Chemistry and Laboratory Medicine 51.10 (2013): n. pag. Web.
    6. Sharma, S.k., Mohammad Tahir, Alladi Mohan, Duncan Smith-Rohrberg, Hemant K. Mishra, and R.m. Pandey. “Diagnostic Accuracy of Ascitic Fluid IFN-γ and Adenosine Deaminase Assays in the Diagnosis of Tuberculous Ascites.” Journal of Interferon & Cytokine Research 26.7 (2006): 484-88. Web.
    Documents
    Enquiry