1,5-Anhydroglucitol
The 1,5-Anhydroglucitol (1,5-AG) assay is used for the quantitation of 1,5-Anhydroglucitol in human serum or plasma.
1,5-Anhydroglucitol (AG; popularly called 1-deoxyglu- cose) has a pyranoid structure, resulting from the deletion of an oxygen from glucose at the anomeric hydroxyl group. This compound is one of the main sugar alcohols in human cerebrospinal fluid and serum. In plasma, the concentration of AG is reduced specifically in diabetes mellitus, thus ninking it useful as a diagnostic marker for the disease. Because 1,5-AG is similar structures with glucose, due to high blood glucose brings the glucose excrete (diabetes), competition with glucose 1,5 – AG, make urine reuptake 1,5-AG emissions increase of serum concentrations, reduced. At this time, the body 1,5-AG storage pool also reduces, reduce blood glucose after improvement, with normally accepted from food supply returned to normal. 1,5-AG can sensitive reaction, blood sugar control as a highly sensitive state-of-the-art of glucose in blood sugar, comprehensive index forecast changes, is determination can also mean days interval of meaningful change. Especially to grasp mild diabetics glucose change is very effective.
Pyranonse oxidase, Hexokinase and ATP regenerative system that can detect the serum 1,5 -AG concentration. The assay utilizes Hexokinase and ATP regenerative system converts glucose to Glucose-6-phosphate, a compound that does not react with Pyranose oxidase. The hydrogen peroxide produced in the oxidation of 1,5AG by Pyranose oxidase is detected with a standard enzymatic color-developing system.
Hexokinase Glucos e + ATP → Glucose-6-phosphate + ADP
Pyruvaste Kinase
PEP + ADP Pyruvate + ATP → Pyruvate + ATP
Pyranose Oxidase
1,5-Anhydroglucitol + O2 → 1,5anhydro-fructose + H2O2
POD
H2O2+ 4-aminoantipyrine/HTIB → H2O +Dye
- Masahiko Yabuchi , etc. CLIN. CHEM 35/10,2039- 2043(1989).
- Kayhleen M , ete. DIABETES CARE.Vol 29, 1214- 1219