γ-Glutamyl transferase Assay Kit (γ-GT/GGT)
For the in vitro quanti-tative determination of γ- glutamyl transferase in human serum or plasma. This product is suitable for manual use, and is also suitable for all automaticanalyzer.
γ-GT plays an important role in amino acid transport in the course of glutathione metabolism. The enzyme present in the serum is mainly of hepato-biliary origin. Increased enzyme activities are found in association with chronic alcoholism, different toxic liver damages, intra- and extrahepatic cholestasis, acute viral hepatitis, pancreatitis, neoplastic diseases of the liver and pancreas myocardial infarction as well as with diabetes mellitus.
γ-GT catalyzes the transfer of glutamic acid to acceptors like glycylglycine. This process releases 5amino-2-nitrobenzoate, which can be measured at 405nm. The increase in absorbance at this wavelength is directly related to the activity of γ -GT.
γ-GT
L-γ-glutamyl-3-carboxy-4 nitroanilide+glycylglycine → L-γ-glutamyl-glycylglycine+5-amino-2nitrobenzoate
- Thomas L. Clinical Laboratory Diagnostics. 1st ed. Frankfurt: TH Books Verlagsgesellschaft;1998.p. 80-6.
- Persijn JP, van der Silk W. A new method for the determination of gamma-glutamyltransferase in serum. J Clin Chem Clin Biochem1976; 14:421-7.
- Szasz G. Gamma-Glutamyltranspeptidase. In: Bergmeyer HU. Methoden der enzymatischen Analyse. Weinheim: Verlag Chemie, 1974. p. 757.