α2-macroglobulin
This product is used on NEPHSTAR® protein analysis system for quantitative determination of human a2-macroglobulin (AMG) in serum
Human a2-macroglobulin (AMG) is a plasma glycoprotein of molecular weight 725,000, containing four identical subunits bound together by -S-S- bonds. This protein binds to a wide variety of proteases, usually resulting in inhibition of the activity of these proteases. Increased levels are reported in nephrotic syndrome, pregnancy, liver diseases, diabetes mellitus, inflammatory diseases, bronchopneumonia, congenital cardiac disease. Decreased levels are reported in fibrinolysis, acute pancreatitis, biliary or renal stones, liver tumors, gastroduodenal ulcers, myocardial infarction.
Immunonephelometry is applied. This method involves measuring the light scattered by insoluble complexes formed by reaction between specific protein in samples and its respective antiserum, and the amount of scattered light is directly proportional to the concentration of the protein under condition that antiserum is in excess. Concentrations are automatically calculated by reference to a calibration curve stored in the instrument.
1. Urdal P, Borch SM, Landaas S, Krutnes MB, Gogstad GO, Hjortdahl P. Rapid immunometric measurement of C-reactive protein in whole blood. Clin Chem 1992;38:580-584.
2. Chambers RE, Whicker JT, Dieppe PA. Acute phase proteins in inflammatory disease. Clin Diagnosis Lab 1988;1:29-37.
3. Morley JJ, Kushner I. Serum C-reactive protein levels in disease. Ann N Y Acad Sci 1982;389:406-18.
4. Lindback S, Heligren U, Julander I, Hanseon LO. The value of C-reactive protein as a marker of bacterial infection in patients with septicaemia, endocarditis and influenza. Scand J Infect Dis 1989;21:543-.9.