α-L-Fucosidase (AFU)
The α-L-fucosidase (AFU) assay kit is for determination of AFU activity in serum samples.
AFU Assay is a single reagent, one step method that has a linear range of 0-300 U/L. The reagent is ready-to-use for both manual and automatic chemistry analyzers. The assay is specific for AFU and has no detectable reaction with other glycosidases. The assay is not affected by serum bilirubin up to 100 mg/dL, hemoglobin up to 200 mg/dL, triglycerides up to 750 mg/dL, or ascorbic acid up to 4.4 mg/dL. The assay has precisions of Intra %CV < 5.1% and Inter CV% <6.2%.
The AFU Assay is based on the enzymatic cleavage of the synthetic substrate 2-chloro- 4-nitrophenyl-α-L-fucopyranoside to α-L-fucoside and 2-chloro-4-nitrophenol, which is quantified by measuring the absorbances at 405 nm in a kinetic fashion. It is a one step assay with a single assay reagent. One unit of AFU is defined as the amount of AFU that cleaves one μmole of 2-chloro-4-nitrophenyl-α-L-fucoside per min at 37°C.
