α-Hydroxybutyrate dehydrogenase Assay Kit (α-HBDH)
For the in vitro quantitative determination of α hydroxybutyrate dehydrogenase activity in serum.
Product Features
Determination of α-HBDH is useful in the diagnosis of myocardial infarction. Elevated α- HBDH can usually be detected within 12 hours of an infarction, with peak values being determined after 48 to 72 hours. Increased serum activity can also be found in the Duchenne form of muscular dystrophy, megaloblastic anemia and widely disseminated metastases.
Assay Principle
HBDH
2-oxobutyrate+NADH+H+→ 2-hydroxybutyrate+NAD+
The oxidation of NADH results in a decrease in absorbance at 340 nm which is proportional to the activity of the enzyme in the sample.
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References
- Rosalki, S.B. and Wilikinson, J.H., Nature 188:1110 (1960).
- Z. Klin. Chem. U. Klin. Biochem. 8:658 (1970).
- Z. Klin. Chem. U. Klin. Biochem. 10:182 (1972).