Insulin Assay
For the quantitative determination of human insulin in serum and plasma by immunoturbidimetric assay
Insulin Assay is a dual liquid stable reagent system which provides reliable results. The method requires only 12 μLof sample, completed in less than 10 minutes, and has excellent correlation within-run existing commercial products with R2 of 0.9997, a slope of 0.8061 and a y intercept of 2.8674. The assay has within-run precision values lower than 1.19% and a between-run precision value of 4.34%.
Latex particles coated with antibody specific to human insulin form immune complexes in the presence of insulin from the sample. The immune complexes cause an increase in light scattering that is pro-portional to the concentration of insulin in the serum or plasma sample. The light scattering is measured by reading turbidity at 600 nm primary, 800 nm secondary. The sample insulin concentration is determined versus insulin calibrators of known concentrations.