Melatonin-Sulfate
Enzyme immunoassay for determination of melatonin sulfate (synonyms: 6-Hydroxymelatonin Sulfate, 6- Sulfatoxymelatonin) in human urine.
Test Principle
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed color is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.
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Refrences
- Kondo, M. Tokura H., Wakamura T., Hyun KJ., Tamotsu S., Morita T., Oishi T., Influence of twilight on diurnal variation of core temperatuer, its nadir, and urinary 6-hydroxymelatonin sulfate during nocturnal sleep and morning drowsiness. Coll. Antropol. 33 (2009) 1: 193-199
- Radwan P., Skrzydlo-Radomanska B., Radwan-Kwiatek K., Burak-Czapiuk B., Strzemecka J. Is melatonin involved in the irritable bowel syndrome? Journal of Physiology and Pharmacology (2009), Suppl 3, 67-70
- Mahlberg R. et al. Normative data on the daily profile of urinary 6-sulfatoxymelatonin in healthy subjects between the ages of 20 and 84. Psychoneuroendocrinology (2006) 31, 634-641
- Kunz et al. Melatonin in Patients with Reduced REM Sleep Duration: Two Randomized Controlled Trials. Journal of Clinical Endocrinology & Metabolism, 89(1): 128-134 (2004)
- Mahlberg et al. Melatonin and age. Somnologie 7 (Suppl. 1): 9-88 (2003)
- Griefahn et al. Melatonin production during childhood and adolescence: a longitudinal study on the excretion of urinary 6-hydroxymelatonin sulfate. J. Pineal Res. 34:26-31 (2003)
- Bortkiewicz et al. The excretion of 6-hydroxymelatonin sulfate in healthy young men exposed to electromagnetic fields emitted by cellular phone – an experimental study. Neuroendocrinology Letters 23(suppl 1):88-91 (2002)
- Kunz et al. Exogenous Melatonin in Periodic Limb Movement Disorder: An Open Trial and a Hypothesis. SLEEP, Vol. 24, No. 2, 183-187, (2001).
- Griefahn et al. Long-Term Stability of 6-Hydroxymelatonin Sulfate in 24-h Urine Samples Stored at –20°C. Endocrine, vol. 15, no. 2, 199-202, July (2001).
- Kunz D et al. Melatonin as a therapy in REM sleep behavior disorder patients: an open-labeled pilot study on the possible influence of melatonin on REM-sleep regulation. Movement Disorders, 14: 507-511 (1999)
- Pfluger DH, Minder CE. Effects of exposure to 16.7 Hz magnetic fields on urinary 6-hydroxymelatonin sulfate excretion of Swiss railway workers. J. Pineal Res., 21: 91-100 (1996)
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