Double Stranded DNA (dsDNA) IgG ELISA
The Calbiotech, Inc. (CBI) dsDNA IgG ELISA Kit is an enzyme linked immunosorbent assay (ELISA) for the detection of IgG antibody to dsDNA in human serum or plasma.
Anti-dsDNA is present in 50 % to 70% of patients with systemic lupus erythematosus (SLE). Circulating DNA/anti-DNA immune complexes are considered to play a part in the pathogenesis of SLE. The presence of anti-dsDNA is one of the diagnostic criteria for SLE. IgG antibodies to dsDNA are considered clinically most useful for the diagnosis and management of SLE. Antibodies to single stranded DNA (ssDNA) and IgM antibodies to DNA are found in a number of other connective diseases, liver diseases, as well as in some normal individuals. ELISA is the method of choice for the screening of anti-dsDNA in-patients with suspected SLE.
Diluted patient serum is added to wells coated with purified dsDNA antigen. Specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of specific antibody in the sample.
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