anti-Titin-Antibody
Enzyme immunoassay for the quantitative determination of anti-Titin antibodies in serum or plasma
Procedure
100 µl 1:101 prediluted patient sera
performance within approximately 2 hours
In about 80 % of patients with Myasthenia gravis alterations of the thymus can be detected. Approximately 10 % of these patients develop thymus neoplasia as thymic epithelial tumor (TET) or thymic carcinoma. An early diagnosis of the thymoma and subsequent thymectomy is decisive for the prognosis of these patients.
Most of the affected patients develop acetylcholine receptor antibodies which can be detected with the ACHRAB-Assay for the diagnosis of myasthenia gravis, as well as autoantibodies against striated muscles, among others antibodies to titin.
Titin is a protein of the striated muscles with an extremely high molecular weight. The immunogenic region of titin is located on a 30 kD protein fragment. Antibodies against this fragment presumably crossreact with the epitopes of the acetylcholine receptors (paraneoplastic myasthenia gravis). The recombinant MGT30 peptide is used in the ELISA for the specific determination of anti-titin antibodies.
The new ELISA assay is clearly superior to the immunofluorescence test on sections of striated human or monkey muscles.
The Anti-Titin ELISA kit uses the microtitre plate format. Recombinant titin fragment (MGT30 peptide) is coated onto the surface of the microwells. Diluted serum specimens are incubated to allow antibodies to titin to bind to the plastic surface. After washing away unbound antibodies and serum constituents, the specific titin antibodies are detected by protein Aperoxidase. The TMB / peroxidase reaction is monitored at 450 nm.
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multiple sclerosis. Lancet 2004; 364(9451): 2106 – 2112 - V. A. Lennon et al. IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. The Journal of Experimental Medicine 2005; 202: 473 – 477
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neuromyelitis optica. Multiple Sclerosis Journal 2012 DOI: 10.1177/1352458512443917 - S. Jarius et al. Testing for antibodies to human aquaporin-4 by ELISA: Sensitivity, specificity and direct comparison with immunohistochemistry. Journal of the Neurological Sciences 2012 320: 32 – 37