Monoamine Oxidase Assay Kit (MAO)
For uantitative determination of MAO activity in patient serum or plasma samples. For use as an valuable detecting the development of liver fibrosis and cirrhosis.
The Monoamine Oxidase (MAO) assay kit is intended for quantitative determination of MAO activity in patient serum or plasma samples. Hepatic fibrosis is characterized by strong accumulation of complex connective tissue proteins such as various types of collagens and proteoglycans. MAO is believed to accelerate the rate of cross-linking reaction of collagen fibers[1]. Elevated levels of serum MAO activity (particularly MAO-B or so-called benzylamine oxidase) are frequently found in patients with hepatic cirrhosis, chronic heart failure, diabetes, and hyperthyroidism[2]. Therefore, measurement of serum MAO may afford a valuable mean of detecting the development of liver fibrosis and cirrhosis.
The reagents of the assay kit are in stable liquid formulation that allows ease of use coupled with enhanced performance characteristics. The MAO assay is based on the enzymatic oxidation of the synthetic substrate benzylamine of MAO to generate benzylaladehyde, ammonia and hydrogen peroxide (H2O2). The Ammonia then reacts with NADH and αKetoglutaric acid in the present of GLD to L-Glutamic acid and NAD+, Concomitant with this coenzyme NADH convert to NAD+, the associate change of absorbance at 340 nm can be directly correlated with the MAO activity (U/L).
MAO
R•CH-NH2 + O2+ H2O → R•CHO+NH3 + H2O2
GLDH
NH3 + α- Ketoglutaric acid + NADH → L-Glutamic acid + H2O + NAD+
- Hare MLC(1928)Tyramine oxidase.I.A new enzyme system in liver. Biochem J 22: 968Y979
- Yu P, Boulton A(1987).”Irreversible inhibition of monoamine oxidase by some components of cigarette smoke”. Life Sci 41(6):675-82. PMID 3613836.