Lactate Dehydrogenase–L Assay Kit (LDH)
For the in vitro quantitative determination of lactate dehydrogenase activity in human serum.
Product Features
The enzyme lactate dehydrogenase (LDH-L) is distributed in tissues particularly heart, liver, muscle, and kidney. The enzyme found in circulation is a mixture of five isoenzymes based on their mobility. Elevated serum levels of LDH-L are found in serum in myocardial infraction, liver disease, renal disease, certain forms of anemia, malignant diseases and progressive muscle dystrophy.
Assay Principle
LDH
L－lactate + NAD+ → pyruvate + NADH + H+
LDH catalyzes the oxidation of lactate to pyruvate, and NAD is reduced to NADH, which can be measured at 340nm.
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References
- Amador E., Dorfman L.E., Wacker W.E., Clin. Chem.,1963; 9: 331.
- Henry, R.J., Clinical Chemistry, Principles and Techniques, 2nd Edition, Harper and Row, p. 819, 1974.
- Tietz, N., Fundamentals of Clinical Chemistry, W.B.Saunders Co., Philadelphia, PA, p. 652, 1976
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