Heart-type Fatty Acid-Binding Protein Assay Kit (H-FABP)
For the quantitative determination of human H-FABP in serum and plasma by immunoturbidimetric assay. For in vitro diagnostic use only
Fatty acid-binding proteins (FABP) are a class of cytoplasmic proteins that bind long chain fatty acids. FABP are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. Following acute myocardial infarction (AMI) the small protein H-FABP is rapidly released into the circulation. So H-FABP is a sensitive newAMI marker.
Sample is reacted with a buffer and anti-FABP coated latex. The formation of the antibody-antigen complex during the reaction results in an increase in turbidity, the extent of which is measured as the amount of light absorbed at 700nm. By constructing a standard curve from the absorbance of the standards,H-FABP concentration of sample can be determined.
- Liao J, Chan CP, Cheung YC, Lu JH, Luo Y, Cautherley GW,Glatz JF, Renneberg R. Human heart-type fatty acid-binding protein for on-site diagnosis of early acute myocardial.
- Kilcullen et al. Heart-type fatty acid-binding protein predicts long-term mortality after acute coronary syndrome and identifies high-risk patients across the range of troponin.