High density lipoprotein cholesterol Assay Kit (HDL-C)
For the in vitro quantitative determination of HDL-C in serum.
High-density lipoproteins (HDL) are one of the major classes of plasma lipoproteins. They are composed of a number of heterogeneous particles, including cholesterol and vary with respect to size and content of lipid and apolipoprotein. HDL serve to remove cholesterol from the peripheral cells to the liver, where the cholesterol is converted to bile acids and excreted into the intestine. An inverse relationship between HDL-cholesterol (HDL-C) levels in serum and the incidence/prevalence of coronary heart disease (CHD) has been demonstrated in a number of epidemiological studies. The importance of HDL-C as a risk factor for CHD is now recognized[1].Accurate measurement of HDL-C is of vital importance when assessing patient risk from CHD. In this diagnostic test kit a method for direct measurement of HDL-C, without sample pretreatment, is presented. Direct measurement gives improved accuracy and reproducibility when compared to precipitation methods.
The direct HDL Cholesterol assay is a homogeneous method for directly measuring serum HDL-C levels without the need for any pretreatment and centrifugation steps. First step, substances with high affinity to LDL, VLDL, and chylomicrons block them involving to enzyme reaction. Second step, special surfactant that selectively accelerates reaction with the enzyme reagent with HDL cholesterol and determining them.
HDL-C → cholestenone + H2O2
POD
2H2O2 + 4-AA+ TOD → purple-blue pigment (λmax=585 nm)