Glucose Assay Kit (GLU)
For the in vitro quantitative determination of glucose in serum.
Product Features
Determination of glucose concentration is important in the diagnosis and treatment disorders of carbohydrate metabolism. Increased levels of glucose are found in diabetes mellitus, hyperparathyroidism, pancreatitis, renal failure. Decreased levels are found in insulinoma, hypothyroidism, hypopituitarism and extensive liver disease.
Assay Principle
According to the equation, the formation rate of NADPH is directly proportionto the glucose concentration.. NADPH has a absorption peak in wavelengh 340nm, the increase rate of absorbance is directly proportion to the GLU concentration of the sample.
GLU + ATP → G6P + ADP
G6PD
G6P + NADP → 6PG + NADPH + H+
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References
- Yanan Zhang Clinical Significance of Serum GlucoseDetermination, Modern Chinese medicine application 2012 6(9)43-45
- Shenyuan Yan, Guangran Yang Hypoglycemia Foreign Medical Archies of Endocrinology 2005 1 (25)70-72
- Xiuming Zhang, jianzhai Li, Ming Wei etc. Mordern Clinical Biochemical[M].Beijing People’s Military Medical Press,2011:84-85.
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