Neutrophil Gelatinase-Associated Lipocalin Assay Kit (NGAL)
For the in vitro quantitative determination of NGAL in urine or plasma samples.
NGAL (Neutrophil Gelatinase-Associated Lipocalin), a 25-kDa glycoprotein covalently bound to gelatinase, it is synthesized and secreted by tubular epithelial cells of the proximal and distal segment. In healthy kidneys, it is barely detectable in either plasma or urine. However, in the setting of acute tubular injury, NGAL undergoes rapid and profound up regulation with large increases in both urine and plasma. Distinct from traditional markers of function such as creatinine, this rapid response enables NGAL to identify injured kidney much earlier than was previously possible. In experimental and clinical studies, NGAL has been investigated extensively and would be one of the most frequently investigated and most promising early biomarkers of AKI. NGAL has been investigated across a range of different clinical settings of AKI, such as after cardiac surgery, in critically ill patients, in patients receiving intravenous contrast media infusion for coronary angiography, and in patients admitted to the emergency department et al.
The antigen-antibody reaction occurs between NGAL in a sample and anti-NGAL antibody which has been coated to latex particles.It results a aggutination. This agglutination is detected as an absorbance change (570 nm), with the magnitude of the change being proportional to the quantity of NGAL in the sample. The actual concentration is then determined by a calibration curve prepared from calibrators of known concentration.
1. Bennett M, Dent CL, Ma Q, et al. Urine NGAL predicts severity of acute kidney injury after cardiac surgery: a prospective study. Clin J Am Soc Nephrol. 2008; 3:665-673.
2. Haase-Fielitz A, Bellomo R, Devarajan P, et al. Novel and conventional serum biomarkers predicting acute kidney injury in adult cardiac surgery—a prospective cohort study. Crit Care Med. 2009;37:553-560.
3. Wheeler DS, Devarajan P, Ma Q, et al. Serum neutrophil gelatinase-associated lipocalin (NGAL) as a marker of acute kidney injury in critically ill children with septic shock. Crit Care Med. 2008;36:1297-1303.d
4. Hirsch R, Dent C, Pfriem H, et al. NGAL is an earlypredictive biomarker of contrast-induced nephropathy in children. Pediatr Nephrol. 2007;22:2089-2095.