Glucose-6-Phosphate Dehydrogenase Assay Kit (G-6-PD)
For quantitative determination of Glucose-6Phosphate Dehydrogenase (G6PD) in human blood.
Glutathione(GSH) is the necessary condition of maintaining the stability of hemoglobin and the integrity of red cell membrane. Glutathione reductase itself needs sufficient amount of NADPH. The level of NADPH is kept sufficient by G6PD. G6PD deficiency can be triggered by some drugs, such as Antimalarial drugs, sulfone drugs, primaquine, sulfa drugs. Low G6PD values are found with G6PD deficiency, certain drugs, fava beans, infections, etc.
Glucose-6-phosphate dehydrogenase catalyzes the first step in the pentose phosphate shunt, oxidizing glucose-6-phosphate (G-6-P) to 6phosphogluconate (6-PG) and reducing NADP to NADPH. Nictotinamide adenine dinucleotide phosphate (NADP) is reduced by G6PD in the presence of G-6-P. The rate of formation of NADPH is proportional to the G6PD activity and is measured colorimetrically as in increase in absorbance at 340nm. Involving the following reaction:
G6PDH
G-6-P + NADP+ ——-→ 6-PG + NADPH + H+