Total Bilirubin Assay Kit (TBil)
For the in vitro quantitative determination of total bilirubin in serum.
Total bilirubin is comprised of direct bilirubin and indirect bilirubin.The total bilirubin are increased in liver cell jaundice, as if acute jaundice hepatitis, critically ill hepatitis, slow live liver, liver cirrhosis and so on. The total bilirubin and the direct bilirubin are also elevated in the blocking jaundice, like biliary duct stone, biliary duct obstruction and so on. The total bilirubin and the indirect bilirubin are also elevated common in hemolytic jaundice.
The bilirubin in acidic conditions reacts with the diazodichloraniline and product the color azo compound, the color diazo compound production quantity is proportional to the concentration of the total bilirubin in the sample.
1. Winsten, S. and Cehely, K. B. (1968) Clin. Chem. 14,107.
2. Tietz, N. (1990) in Clinical Guide To Laboratory Tests.Ed. W. B. Saunders. CP Philadelphia, PA. p90.