Direct Bilirubin Assay Kit (DBil)
Product Features
Each types hepatitis, liver cirrhosis, the blocking jaundice obviously direct bilirubin increase, is more sensitive than the transaminase. It is especially suits in the chronic hepatitis prognosis appraisal and the early liver cirrhosis diagnosis. When serious anemia in the blood serum or the blood plasma direct bilirubin decreases.
Assay Principle
When a sample is mixed with the reagent containing the detergent and the vanadate, at around pH 3, the direct bilirubin in the sample is oxidized to biliverdin. This causes the absorbance of yellow, specific to bilirubin, to decrease. Therefore, the total bilirubin concentration in the sample can be obtained by measuring the absorbances before and after the vanadate oxidation.
vanadicacid
bilirubin → biliverdin
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References
- Lott JA, Doumas BT. Direct and total bilirubin tests: contemporary problems. Clin Chem, 1993,39(4):641-647.
- Shetty K, Rybicki L, Carey WD. The Child Pugh classification as a prognostic indicator for survival in primary sclerosing cholangitis. Hepatol, 1996,25(5):1049-1053.
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