Total protein Assay Kit (TP)
For the quantitative in vitro determination of total protein in human serum.
The assay kit is for determination of serum total proteins (TP). Proteins are constituents of muscle, enzymes, hormones and several other key functional and structural entities in the body. They are involved in the maintenance of the normal distribution of water between blood and the tissues. Consisting mainly of albumin and globulin the fractions vary independently and widely in diseases. Increased levels are found mainly in dehydration. Decreased levels are found mainly in malnutrition, impaired synthesis, protein losses as in hemorrhage or excessive protein catabolism.
Total proteins bind with the Cu2+ in a buffered medium to form a coloured complex. The intensity of the colour formed is directly proportional to the amount of total proteins present in the sample at 546 nm.
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