Urea Assay Kit (UREA)
For the in vitro quantitative determination of Urea in serum.
Urea is the final degradation product of protein and amino acid metabolism. In protein catabolism the proteins are broken down to amino acids and deaminated. The ammonia formed in this process is metabolized to urea in the liver. This is the most important catabolic pathway for eliminating excess nitrogen in the human body. The test is frequently used for the differential diagnosis of prerenal hyperuremia (cardiac decompensation, water depletion increased protein catabolism), renal hyperuremia (glomerulonephritis, chronic nephritis. polycystic kidney, nephrosclerosis, tubular necrosis) and postrenal hyperuremia (obstructions of the urinary tract).
The enzymatic method involves a series of coupled enzymatic reactions. Urea in the specimen is converted to ammonium and carbon dioxide by urease, and then the product ammonium is assayed by glutamate dehydrogenase (GLDH).
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