Bicarbonate Assay Kit (CO2)
For the in vitro quantitative determination of CO2 in serum or plasma.
Product Features
Increased blood CO2, (hypercapnia) causes respiratory acidosis. CO2 rises with decreased alveolar ventilation due to diseases of the lungs or bronchial tree, or breathing CO2 enriched air. Depression of the overall lung capacity by certain drugs may lead to retention of CO
Assay Principle
PEP-C+Mg2+
Phosphoenolpyruvate + HCO3– → oxaloacetate + H2PO4
MDH
oxaloacetate + NADH + H+ → malate + NAD+
The reduction in absorbance at 340 nm caused by the oxidation of NADH is proportional to the bicarbonate concentrationin the sample.
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References
- Tietz, N. N., et al “Textbook of Clinical Chemistry” W. B. Saunders Co., 1986; 1172-1253.
- Jacobs, N., et al “Laboratory Test Handbook” 2nd. ed., Williams and Wilkins 1990.
- Forrester, R.L., Wataji, L.J., Silverman, D.A., Pierre K.J., Clin, Chem. 1976; 22/2: 243-245.
- Young D.S., Effects of Drugs on Chemical Laboratory Tests, 3rd ed., AACC Press 1990.
- Norris, K.A., Atkinson, A.R., Smith, W.G., Clin. Chem. 1975; 21/8: 1093 – 1101.
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