Alkaline phosphatase Assay Kit (ALP)
For the in vitro quantitative determination of alkaline phosphatase (ALP) in serum.
Alkaline phosphatase is a membrane-bound enzyme which is present in most tissues. It has three different isoenzymes derived from small intestine-placentabone/liver/kidney. It is a dimer molecule containing Zn2+ ions, which play a role in the maintenance of structure and catalysis. The enzyme found in human serum is derived from bone, liver and small intestine. During pregnancy the enzyme from the placenta dominates (it is heat stable at 65°C). In the past the isoenzymes were separated using various inhibitors and heat. The role of electrophoresis is growing in determining the concentrations. The increase in enzyme activity is prevalent in various hepatic and bone decrease states. The level is also increased in certain diseases of the thyroid gland, intestinal tract and in several bacterial infection.
Under alkaline condition, colorless p-nitrophenol is converted to 4-nitrophenoxide, which develops a very intense yellow color. Its intensity is proportional to the activity of alkaline phosphatase. Alkaline phosphatase
p-nitrophenyl phosphate + H2O → p-nitrophenol + phosphate
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