Albumin Assay Kit (ALB)
For the in vitro quantitative determination of Albumin in serum.
Albumin consists of approximately 60% of the total proteins in the body, the other major part being globulin. It is synthesized in the liver and maintains the osmotic pressure in blood. Albumin also helps in the transportation of drugs, hormones and enzymes. Elevated levels are rarely seen and are usually associated with dehydration. Decreased levels are seen in liver diseases (Hepatitis, Cirrhosis). Malnutrition, kidney disorders, increased fluid loss during extensive burns and decreased absorption in gastro-intestinal diseases.
Albumin binds with the dye Bromocresol Green in a buffered medium to form a green coloured complex. The intensity of the colour formed is directly proportional to the amount of albumin present in the sample at 600 nm.
- Grant G.H., et al Amino Acids and Proteins;Fundamentals of Clinical Chemistry, Tietz N.W.Editor,Third Edition, WB Saunders Company PhiladelphiaUSA, 328-329, 1987
- Doumas, B.T., Watson, W.A., Biggs, H.G., Clin. Chim.Acta. 1971; 31: 87.