N-acetyl-β-D-glucosaminidase (NAG)
The N-Acetyl-β-D-glucosaminidase (NAG) assay kit is for determination of NAG in urine samples.
N-Acetyl-β-D-glucosaminidase (NAG) Assay is ready-to-use for manual methods and adaptable for analyzers. The assay is specific for NAG and has excellent sensitivity with a linear range up to 200 IU/L² and excellent precision with a CV% of <5%. There is no significant interference from hemoglobin or albumin. Interference from bilirubin occurs only at levels higher than 5 mg/dL.
The reagents of the assay kit are in stable liquid formulation that allows ease of use coupled with enhanced performance characteristics. NAG hydrolyses 2-methoxy-4-(2’nitrovinyl)-phenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (MNP-GlcNAc) to 2-methoxy-4-(2′-nitrovinyl)-phenol product. The product formation is detected by development of color at 505nm upon addition of an alkaline buffer.
