Apolipoprotein B (Apo B)
Apolipoprotein B Assay is intended for the quantitative in vitro determination of Apolipoprotein B (Apo B) in serum. It can be used as an aid for assessing the risk of coronary artery disease.
Apo B Assay has a linear range of 3.6 – 240 mg/dL with good correlation with existing FDA approved product with r2 values >0.99. The Diazyme Apo B Assay is not interfered by the following substances at the indicated concentrations: triglycerides at 1000 mg/dL, ascorbic acid at 10 μM, bilirubin at 40 mg/dL, bilirubin conjugate at 40 mg/dL, and hemoglobin at 1000 mg/dL.
This method is based on the reaction of a sample containing human Apo B and a specific antiserum to form an insoluble complex which can be measured turbidimetrically at 340 nm. By constructing a standard curve from the absorbance of standards the concentration of apo B in the samples can be determined.
