Leptin Human ELISA, Clinical Range
Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa protein consisting of 146 amino acid residues. Leptin is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, particularly hypothalamus, affecting food intake. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned. Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients.
Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice. Endogeneous leptin can normalize their body weight. In contrast, high levels of leptin in obese human subjects point to an insensitivity to endogeneous leptin. Other factors in addition to the amount of body fat appear to regulate leptin action: insulin, glucocorticoids, catecholamines and sex hormones. Studies have shown that leptin may be
linked to reproductive function.
The RD191001100 Human Leptin ELISA, Clinical Range is a sandwich enzyme immunoassay for the quantitative measurement of human leptin in serum and plasma.
In the BioVendor Human Leptin ELISA Clinical Range, Standards, Quality Controls and samples are incubated in microplate wells pre-coated with polyclonal anti-human leptin antibody. After 60 minutes incubation and washing, polyclonal anti-human leptin antibody, conjugated with horseradish peroxidase (HRP) is added to the wells and incubated for 60 minutes with captured leptin. Following another washing step, the remaining HRP conjugate is allowed to react with the Substrate Solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of leptin. A standard curve is constructed by plotting absorbance values against concentrations of Standards, and concentrations of unknown samples are determined using this standard curve.
Intended use
Clinical Application
Test principle
Summary of protocol
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