Interleukin-6 Rat ELISA
Interleukin-6 is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis, and it may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide or viral infections.
Research topic
Animal studies, Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Cell culture supernatant
Sample Requirements
50 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
31.0–2000 pg/ml
Limit of Detection
12.0 pg/ml
Intra-assay (Within-Run)
CV = 5.0%
Inter-assay (Run-to-Run)
CV = 10.0%
Dilutation Linearity
97,00%
– Peric Kacarevic Z, Snajder D, Maric A, Bijelic N, Cvijanovic O, Domitrovic R, Radic R. High-fat diet induced changes in lumbar vertebra of the male rat offsprings. Acta Histochem. 2016 Aug 27;