IgM Rheumatoid Factor Mouse ELISA
Rheumatoid factors are autoantibodies against Fc region of of IgG and is found in 70-80% of patients suffering from chronic rheumatoid arthritis, and are considered to be closely related to its pathological symdrome. Experimental animal models with spontaneous autoimmune diseases similar to those in humans, and animals with artificially-induced inflammation have been used to elucidate the mechanism of autoimmune diseases and to search for potential new remedies. A representative model animal of spontaneous autoimmune diseases is MRL/lpr mouse. As MRL/lpr shows high incidence of lymph node tumor, nephritis, angitis, and arthritis, this animal strain is useful for studies of the mechanism of human autoimmune diseases including rheumatoid arthritis. Autoantibodies found in MRL/lpr serum are IgG type rheumatoid factor, IgM type rheumatoid factor, anti-ssDNA antibodies, anti-dsDNA antibodies, and anti-Sm antibody, etc. This kit enables quantification and comparison of IgM type rheumatoid factor with a calibration curve using standard antibody preparation.
Type
Direct ELISA, HRP-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Citrate
Sample Requirements
1 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
15.6–1 000 mU/ml
Limit of Detection
0.78 mU/ml
– Kawane K, Ohtani M, Miwa K, Kizawa T, Kanbara Y, Yoshioka Y, Yoshikawa H, Nagata S. Chronic polyarthritis caused by mammalian DNA that escapes from degradation in macrophages. Nature. 2006 Oct 26;443 (7114):998-1002
– Kikukawa T, Kojima M, Abe C. A novel assay kits for autoantibodies rate on spontaneous autoimmune model mice. Jap J Inflammation. 2000;20: 697-701
– Kobayashi K, Suda T, Nan-Ya K, Sakaguchi N, Sakaguchi S, Miki I. Cytokine production profile of splenocytes derived from zymosan A-treated SKG mice developing arthritis. Inflamm Res. 2006 Aug;55 (8):335-41
– Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H. Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment. Int Immunol. 2005 Oct;17 (10):1277-82
– Tsuji F, Yoshimi M, Katsuta O, Takai M, Ishihara K, Aono H. Point mutation of tyrosine 759 of the IL-6 family cytokine receptor, gp130, augments collagen-induced arthritis in DBA/1J mice. BMC Musculoskelet Disord. 2009;10:23
– Yamanishi S, Iizumi T, Watanabe E, Shimizu M, Kamiya S, Nagata K, Kumagai Y, Fukunaga Y, Takahashi H. Implications for induction of autoimmunity via activation of B-1 cells by Helicobacter pylori urease. Infect Immun. 2006 Jan;74 (1):248-56