Ferritin Human ELISA
Ferritin, a 450 kDa protein, is an iron storage protein containing up to 4,500 iron atoms in a molecule. It is found in many tissues but has its highest concentration in the spleen, liver, red marrow, small intestinal mucosa and muscle. The serum level of ferritin is an indirect measure of the amount of iron in the blood making it an important marker in anemia and iron metabolism research. Liver disease or malignant tumors can cause an abnormal elevation of serum ferritin levels.
Research topic
Iron metabolism, Oncology
Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum
Sample Requirements
20 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
10–1500 ng/ml
Limit of Detection
0.44 ng/ml
Intra-assay (Within-Run)
CV = 7.3%
Inter-assay (Run-to-Run)
CV = 4.5%
Spiking Recovery
94,40%
Dilutation Linearity
101,30%
– Scibior A, Adamczyk A, Golebiowska D, Niedzwiecka I. Effect of 12-week vanadate and magnesium co-administration on chosen haematological parameters as well as on some indices of iron and copper metabolism and biomarkers of oxidative stress in rats. Environ Toxicol Pharmacol. 2012 Sep;34 (2):235-52
– Stefanova KI, Delcheva GT, Maneva AI, Batalov AZ, Geneva-Popova MG, Karalilova RV, Simitchiev KK. Pathobiochemical Mechanisms Relating Iron Homeostasis to Parameters of Inflammatory Activity and Autoimmune Disorders in Rheumatoid Arthritis. Folia Med (Plovdiv). 2016 Dec 1;58(4):257-263. doi: 10.1515/folmed-2016-0040