Cystatin C Rat ELISA
Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36 kDa and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer’s disease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.
Features
- It is intended for research use only.
- The total assay time is less than 3.5 hours.
- The kit measures total Cystatin C in rat serum.
- Assay format is 96 wells.
- Quality Controls are animal serum based. No human sera are used.
- Standard is recombinant protein based.
- Components of the kit are provided ready to use, concentrated or lyophilized.
Research topic
Animal studies, Neural tissue markers, Renal disease
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.78–25 ng/ml
Limit of Detection
0.008 ng/ml
Intra-assay (Within-Run)
CV = 4.7%
Inter-assay (Run-to-Run)
CV = 4.2%
Spiking Recovery
104,70%
Dilutation Linearity
102,80%
Crossreactivity
| bovine | Non-detectable |
|---|---|
| cat | Non-detectable |
| goat | Non-detectable |
| hamster | Non-detectable |
| horse | Non-detectable |
| dog | Non-detectable |
| monkey | Non-detectable |
| mouse | Yes (recommended dilution 1:100) |
| pig | Non-detectable |
| rabbit | Non-detectable |
| rat | Yes |
| sheep | Non-detectable |
| human | Non-detectable |
| chicken | Not tested |
– Peng ZY, Zhou F, Kellum JA. Cross-species validation of cell cycle arrest markers for acute kidney injury in the rat during sepsis. Intensive Care Med Exp. 2016 Dec;4 (1):12
– Tsuda H, Isaka Y, Takahara S, Horio M. Discrepancy between serum levels of low molecular weight proteins in acute kidney injury model rats with bilateral ureteral obstruction and bilateral nephrectomy. Clin Exp Nephrol. 2009 Dec;13 (6):567-70