Cystatin C Mouse ELISA
Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36 kDa and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer’s disease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.
Features
- It is intended for research use only.
- The total assay time is less than 3.5 hours.
- The kit measures total Cystatin C in mouse serum.
- Assay format is 96 wells.
- Quality Controls are animal serum based. No human sera are used.
- Standard is recombinant protein based.
- Components of the kit are provided ready to use, concentrated or lyophilized.
Research topic
Animal studies, Neural tissue markers, Renal disease
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum
Sample Requirements
5 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.156–10 ng/ml
Limit of Detection
0.04 ng/ml
Intra-assay (Within-Run)
n = 8; CV = 7.1%
Inter-assay (Run-to-Run)
n = 5; CV = 8.2%
Spiking Recovery
88,70%
Dilutation Linearity
104,80%
Crossreactivity
| bovine | Non-detectable |
|---|---|
| cat | Non-detectable |
| goat | Non-detectable |
| hamster | Yes (recommended dilution 1:100) |
| horse | Non-detectable |
| dog | Non-detectable |
| monkey | Non-detectable |
| mouse | Yes |
| pig | Non-detectable |
| rabbit | Non-detectable |
| rat | Yes (recommended dilution 1:200) |
| sheep | Non-detectable |
| human | Non-detectable |
| chicken | Not tested |
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– Jang HR, Gandolfo MT, Ko GJ, Satpute SR, Racusen L, Rabb H. B cells limit repair after ischemic acute kidney injury. J Am Soc Nephrol. 2010 Apr;21 (4):654-65
– Rieger A, Kemter E, Kumar S, Popper B, Aigner B, Wolf E, Wanke R, Blutke A. Missense Mutation of POU Domain Class 3 Transcription Factor 3 in Pou3f3L423P Mice Causes Reduced Nephron Number and Impaired Development of the Thick Ascending Limb of the Loop of Henle. PLoS One. 2016;11 (7):e0158977