TruQuick™ Influenza A/B

The TruQuick Influenza A/B is a rapid chromatographic immunoassay for the qualitative detection of influenza A and B antigens in nasopharyngeal swab, throat swab or nasal aspirate specimens. It is intended to aid in the rapid differential diagnosis of influenza A and B viral infections.


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Product Catalog No: TQ5820 Pack Size: 25 Tests0

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Summary

Influenza (commonly known as ‘flu’) is a highly contagious, acute viral infection of the respiratory tract. It is a communicable disease easily transmitted through the coughing and sneezing of aerosolized droplets containing live virus. 1 Influenza outbreaks occur each year during the fall and winter months. Type A viruses are typically more prevalent than type B viruses and are associated with most serious influenza epidemics, while type B infections are usually milder. The gold standard of laboratory diagnosis is 14-day cell culture with one of a variety of cell lines that can support the growth of influenza virus. 2 Cell culture has limited clinical utility, as results are obtained too late in the clinical course for effective patient intervention. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) is a newer method that is generally more sensitive than culture with improved detection rates over culture of 2-23%. 3 However, RT-PCR is expensive, complex and must be performed in specialized laboratories. The TruQuick Influenza A/B (Swab/Nasal Aspirate) qualitatively detects the presence of Influenza A and/or Influenza B antigen in nasopharyngeal swab or throat swab or nasal aspirate specimens, providing results within 15 minutes. The test uses antibodies specific for Influenza A and Influenza B to selectively detect Influenza A and Influenza B antigen in nasopharyngeal swab, throat swab or nasal aspirate specimens.

Test Procedure
  1. Remove the Test Cassette from the sealed foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed immediately after opening the foil pouch.
  2. Place the Extraction Tube in the workstation. Hold the Extraction Reagent bottle upside down vertically. Squeeze the bottle and let the solution drop into the Extraction Tube freely without touching the edge of the tube. Add 10 drops of solution (approx. 400 μL) to the Extraction Tube. See illustration 1.
  3. Place the swab specimen in the Extraction Tube. Rotate the swab for approximately 10 seconds while pressing the head against the inside of the tube to release the antigen in the swab. See illustration 2.
  4. Remove the swab while squeezing the swab head against the inside of the Extraction Tube as it is removed to expel as much liquid as possible from the swab. Discard the swab in accordance with local biohazard waste disposal protocol. See illustration 3.
  5. Fit the dropper tip on top of the Extraction Tube. Place the Test Cassette on a clean and level surface. See illustration 4.
  6. Add three drops of the solution (approx.120 μL) to the sample well and then start the timer. Read the result at 15 minutes. Do not interpret the result after 20 minutes.
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References
  1. Williams KM, Jackson MA, Hamilton M. Rapid diagnostic testing for URIs in children; Impact on physician decision making and cost. Infec Med. 2002;19(3):109-111.
  2. Betts RF. Influenza virus. In Mandell GL, Douglas RG Jr, Bennett JE. (ed.), Principle and practice of infectious diseases, 4th ed. Churchill Livingstone, Inc., New York, N.Y. 1995;p. 1546-1567.
  3. WHO recommendations on the use of rapid testing for influenza diagnosis, WHO. July 2005.
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