Adenovirus Ag
Adenovirus Ag ELISA is for measurement of Adenovirus in faecal samples.
Adenovirus is the causative agent of respiratory tract infections, conjunctivitis or enteritis. Adenovirus infections are spread via faecal-oral transmission or aerosols (1). Most infections are mild to moderate and do not last longer than one week.
Adenovirus Ag ELISA is a one-step enzyme immunoassay on the basis of monoclonal antibodies against an epitope of the capsid protein hexon, common to all human pathogenic Adenovirus serotypes. Diluted stool specimens and horseradish peroxidase (HRP) labelled monoclonal anti-Adenovirus-antibodies are dispensed simultaneously into the wells of a microtitration plate coated with monoclonal anti-Adenovirusantibodies.
After an incubation time of 60 min at room temperature (RT) unbound components are removed by a washing step.
HRP converts the subsequently added colorless substrate solution of 3,3’,5,5’-Tetramethylbenzidine (TMB) within a 10 min reaction time at room temperature protected from light into a blue product. The enzyme reaction is terminated by sulphuric acid dispensed into the wells turning the solution from blue to yellow. The optical density (OD) of the solution read at 450/≥620 nm is directly proportional to the specifically bound amount of Adenovirus.
Considering the cut-off value results are interpreted as high or low.
- 1. Mentel, R. und Döhner, L. (1996): „Humane Adenoviren.“ Diagnostische Bibliothek Band 1 Virusdiagnostik, Hrsg. Tomas Porstmann, Blackwell Wissenschafts-Verlag Berlin, Wien 1996, S. 103-114
- 2. Schoenemann W. (1988): “Bedeutung von Adenovirusinfektionen im Säuglings- und Kleinkindesalter,” Monatsschrift Kinderheilkunde 136: 680-685
- 3. August, M.J. and Warford, A.L.(1987): „Evaluation of a Commercial Monoclonal Antibody for Detection of Adenovirus Antigen.” Journal Of Clinical Microbiology, Vol. 25, No. 11: 2233-2235