Creatine kinase Assay Kit (CK-NAC)
This reagent is intended for the in vitro quantita-tive determination of creatine kinase in human serum. This product is suitable for manual use, and is also suitable for all automatic analyzer.
Serum creatine kinase (CK) levels have proven valuable in the assessment of cardiac and skeletal muscle diseases, including myocardial infarction and muscular dystrophy. There may also be an increase in CK values associated with diseases of the central nervous system. Diseases of the thyroid show an inverse relationship to CK values. A combined analysis of creatine kinase and lactate dehydrogenase isoenzymes provides a definitive diagnosis of acute myocardial infarction.
Creatine kinase
reatine phosphate+ADP → creatine + ATP
Hexokinase
ATP + glucose → ADP + glucose-6-phosphate
G-6-P-D-H
glucose-6-phosphate + NAD → 6-phosphogluconate + NADH+H+
CK specifically catalyzes thetransphosphorylati-on of ADP to ATP. Through a series of coupled enzymatic reactions, NADH is produced at a rate directly proportional to the CK activity and is measured at 340 nm.
- Tietz, N. W, Textbook of Clinical Chemistry, W.B. SaundersCo.,Philadelphia, 1986, p. 678-6863.
- Szasz, G., et al. Clin. Chem 1976; 22: 650.
- Recommendations of the German Society for Clinical Chemistry.Standardization of methods for the estimation of enzyme activities in biological fluids: Standard method for the determination of creatine kinase activi-ty. J Clin Chem Clin Biochem 1977; 15:255-60.