TruQuick™ Toxo IgG/IgM

TruQuick TOXO IgG/IgM is a lateral flow immunoassay for the simultaneous detection and differentiation of IgM anti- T. gondii and IgG anti-T. gondii in serum or plasma. This kit is intended to be used as a screening test and as an aid in the diagnosis of infection with T. gondii. Any reactive specimen with the TruQuick TOXO IgG/IgM must be confirmed with alternative testing method(s) and clinical findings.


Catalog No Size
Product Catalog No: TQ1240 Pack Size: 40 Tests

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Summary

T. gondii is an obligate intracellular protozoan parasite with a worldwide distribution.1, 2 Serological data indicates that approximately 30% of the population of most industrialized nations is chronically infected with the organism.3 A variety of serologic tests for antibodies to T. gondii have been used as an aid in diagnosis of acute infection and to assess previous exposure to the organism. These tests are the Sabin-Feldman dye test, direct agglutination, indirect hemagglutination, latex agglutination, indirect immunofluorescence, and ELISA.4-7 Recently, lateral flow chromatographic immunoassays, such as The TruQuick TOXO IgG/IgM were introduced into the clinic for the serodiagnosis of T. gondii infection.

Test Procedure
  • Bring the pouch to room temperature before opening it. Remove the Test Cassette from the sealed pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
  • Place the Test Cassette on a clean and level surface. Hold the dropper vertically, draw the specimen up to the Fill Line as shown in illustration below (approximately 10 μL). Transfer the specimen to the specimen well, then add 2 drops of Buffer (approximately 80 μL) and start the timer. See the illustration below.
  • Wait for the colored line(s) to appear. The result should be read at 15 minutes. Do not interpret results after 20 minutes.
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References
  • Krick JA, Remington JS. Toxoplasmosis in the adult: An overview. New Eng J Med. 1978;298:550-553.
  • Anderson SE, Remington JS. The diagnosis of Toxoplasmosis. So Med J. 1975;68:1433- 1443.
  • Wilson CB, Remington JS, Stagno S, Reynolds DW. Development of adverse sequelae in children born with subclinical congenital Toxoplasma infection. Pediatrics. 1980;66:767-774.
  • Berrebi A, et al. Termination of pregnancy for maternal Toxoplasmosis. Lancet.1994;344:36- 9.
  • Fraser KB, Shirodaira PV, Stanford CF. Fluorescent staining and human IgM. Br Med J 1971;3:707.
  • Pyndiah N, Krech U, Price P, Wilhelm J. Simplified chromatographic separation of immunoglobulin M from G and its application to Toxoplasma indirect immunofluorescence. J Clin Micro. 1979;9:170-174.
  • Montoya JG, Rosso F. Diagnosis and management of Toxoplasmosis. Clin Perinatol. 2005;32(3):705-26.
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