Total Bile Acids (Enzyme Cycling Method)

Total Bile Acids Assay Kit is intended for the in vitro quantitative determination of total bile acids (TBA) in human serum.

Regulatery Status: FDA 510(k), CE

Product Catalog Number Packaging Method/Format
Product Catalog No: Kit (400 Tests) Pack Size: DZ042A-K Pack Size: R1: 2 x 60 mL
R2: 2 x 20 mL
Cal: 1 x 2 mL vial
(Calibrator Included)
Pack Size: Enzymatic, Endpoint,
Liquid Stable
Product Catalog No: Kit (400 Tests) Pack Size: DZ042A-KB1 Pack Size: R1: 2 x 60 mL
R2: 2 x 20 mL
Product Catalog No: Kit (400 Tests) Pack Size: DZ042A-KY1 Pack Size: R1: 2 x 60 mL
R2: 2 x 20 mL
Product Catalog No: Calibrator Pack Size: DZ042A-CAL Pack Size: Cal: 1 x 2 mL vial
Pack Size:
Product Catalog No: Control Pack Size: DZ042A-C1V Pack Size: DZ042A-C1V
Product Catalog No: Control Pack Size: DZ042A-CON Pack Size: Con: 2 x 1 mL vial

Category:
Product Features

Total Bile Acids Assay is a liquid stable system, ready to use for both manual methods and is adaptable for many automated chemistry analyzers. The assay has excellent sensitivity with a linear range from 0 – 180 μM. Unique enzyme cycling method amplifies signal for accurate and fast measurement using a small sample up to 50% less than conventional NBT methods making it an excellent choice. The assay has excellent precision with Intra-Assay Precision CV% of <4% and Inter-Assay Precision CV% of <3%. The following substances normally present in serum produced less than 10% deviation at the listed concentrations: Triglycerides at 750 mg/dL, Ascorbic acid at 50 mg/dL, Bilirubin at 50 mg/dL and Hemoglobin at 500 mg/dL. Packaged for optimal operator convenience reagent transfer can be eliminated for most chemistry systems with instrument specific packaging options including RocheTM Hitachi 917 series, Beckman AU (400/600/640/680), Beckman Synchron CX, and DXC.a

Assay Principle

The reagents of the assay kit are in a stable liquid formulation that allows for ease of use coupled with enhanced performance characteristics. In the presence of Thio-NAD, the enzyme 3-α-hydroxysteroid dehydrogenase (3- α-HSD) converts bile acids to 3-keto steroids and Thio-NADH. The reaction is reversible and 3-α-HSD can convert 3-keto steroids and Thio- NADH to bile acids and Thio-NAD. In the presence of excess NADH, the enzyme cycling occurs efficiently and the rate of formation of Thio-NADH is determined by measuring specific change of absorbance at 405 nm.

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