Retinol Binding Protein (RBP) ELISA
The DRG RBP ELISA is intended for measurement of free Retinol-binding protein (RBP)/RBP4 as well as RBP4 complexed with transthyretin in plasma, serum and urine.
This Enzyme-Linked Immunosorbent Assay (ELISA) can be used measurement of Retinol-binding protein (RBP)/RBP4 in plasma, serum and urine.
In a first incubation step, RBP/RBP4 in the samples is bound to polyclonal rabbit anti RBP/RBP4 antibodies, immobilized on the microtitre plate. A peroxidase-conjugated anti RBP/RBP4 antibody is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. A dose response curve of absorbance unit (optical density at 450 nm) vs. concentration is generated using the values obtained from standard. RBP/RBP4 present in the specimen samples is determined directly from this curve.
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