Renin is a Protein (Mw of 37 kDa) that belongs to the aspartic acid protease family of enzymes. Renin regulates the mean arterial blood pressure of the human body by the cleavage of angiotensinogen is processed by angiotensin converting enzyme (ACE) to active octapeptide angiotensin-2. Renin is produced constitutively as prorenin (inactive precursor with 386 amino acids) in juxtaglomerular cells of the kidney. In response to low blood pressure, low sodium chloride, or activity of the sympathetic nervous system, active renin can be released either from a depot in the kidney or generated from prorenin by cleavage of 46 amino acids of N-Terminus of prorenin. The blood concentration of prorenin is approx. 10-fold higher than active renin.
The DRG Aldosterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the of sandwich principle.
The microtiter wells are coated with a monoclonal (mouse) antibody directed towards a unique antigenic site of the renin molecule. An aliquot of patient samples containing endogenous active renin is incubated in the coated well with assay buffer. After incubation, the unbound conjugate is washed.