The IAA-ELISA is an enzyme-linked immunosorbant assay (ELISA) for IAA measurement against human insulin. It is simple to use and does not require the use of radioactive materials.

Regulatery Status: RUO
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Product Catalog No: EIA-1593 Pack Size: 96 Wells

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Test Principle

Human insulin is immobilized onto microwells. The reference, high, and low controls, and diluted specimen serum samples are added to the appropriate microwells. Human IgG specific antibodies to insulin in the serum sample and controls bind to the insulin molecules on the microwells. After washing off unreacted serum materials, an enzyme (alkaline phosphatase) labeled goat antibody specific to human IgG is added to the antigen-antibody complex. After thorough washing to remove the unbound enzyme, a substrate (PNPP) solution is added and the color development is measured spectrophotometrically. The intensity of the color is directly proportional to the concentration of IAA in the sample. Two quality controls (high and low) are provided to monitor and validate assay results.

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References
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  • Goldman, J., D. Baldwin, A.H. Rubenstein, D.D. Klink, W.G. Blackard, L.K. Fisher, T.F. Roe, and J.J. Schnure (1979). Characterization of circulating insulin and proinsulin-binding antibodies in autoimmune hypoglycemia. J. Clin. Invest., 63:1050-1059.
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  • Palmer, J.P., C.M. Asplin, P. Clemens, K. Lyen, O. Tatpati, P.K. Raghu, and T.L. Paquette (1983). Insulin antibodies in insulin-dependent diabetics before insulin treatment. Science, 222:1337-1339.
  • Palmer, J.P., C.M. Asplin, P.K. Raghu, P. Clemens, K. Lyen, O. Tatpati, B. McKnight, T.L. Paquette, M. Sperling, L. Baker, and R. Guthrie (1986). Anti-insulin antibodies in insulin-dependent diabetes before insulin treatment – a new marker for autoimmune beta cell damage? Pediatr. Adolesc. Endocrinol., 15:111-116.
  • Atkinson, M.A., N.K. Maclaren, W.J. Riley, W.E. Winter, D.D. Fisk, and R.P. Spillare (1986). Are insulin antibodies markers for insulin-dependent diabetes mellitus? Diabetes, 35:894-898.
  • Karjalainen, J., M. Knip, A. Mustonen, J. Ilonen, and H.K. Akerblom (1986). Relation between insulin antibody and complement-fixing islet cell antibody at clinical diagnosis of IDDM. Diabetes, 35:620-622.
  • McEvoy, R.C., M.E. Witt, F. Ginsberg-Fellner, and P. Rubinstein (1986). Anti-insulin antibodies in children with type I diabetes mellitus; genetic regulation of production and presence at diagnosis before insulin replacement. Diabetes, 35:634-641.
  • Arslanian, S.A., D.J. Becker, B. Rabin, R. Atchison, M. Eberhardt, D. Cavender, J. Dorman, and A.L. Drash (1985). Correlates of insulin antibodies in newly diagnosed children with insulin-dependent diabetes before insulin therapy. Diabetes, 34:926-930.
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