Homocysteine
The Homocysteine ELISA is intended for measurement of total L-homocysteine in human serum or plasma.
Test Principle
The Homocysteine ELISA is an enzyme immunoassay for the determination of Hcy in blood.[16] Protein-bound Hcy is reduced to free Hcy and enzymatically converted to S-adenosyl-L-homocysteine (SAH) in a separate procedure prior to the immunoassay.[17] The enzyme is specific for the L-form of homocysteine, which is the only form present in the blood.
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References
- Malinow, MR. Plasma homocyst(e)ine and arterial occlusive diseases: A mini-review. Clin Chem 1995;41:173-176.
- Ueland PM. Homocysteine species as components of plasma redox thiol status. Clin Chem 1995;41:340-342.
- Perry IJ, Refsum H, Morris RW, Ebrahim SB, Ueland PM, Shaper AG. Prospective study of serum total homocysteine concentration and risk of stroke in middle-aged British men. The Lancet 1995;346:1395-1398.
- Finkelstein JD. Methionine metabolism in mammals. J Nutr Biochem 1990;1:228-237.
- Mudd SH, Levy HL, Skovby F. Disorder of transsulfuration. In: C.R. Scriver, A.L. Beaudet, W.S. Sly, et al. eds. The metabolic basis of inherited disease New York: McGraw-Hill; 1995:1297-1327.
- Clarke R, Daly L, Robinson K, Naughten E, Cahalane S, Fowler B, Graham I. Hyperhomocysteinemia: An independent risk factor for vascular disease. N Engl J Med 1991;324:1149-1155.
- Deloughery TG, Evans A, Sadeghi A, McWilliams J, Henner WD, Taylor LM Jr, Press RD. Common mutation in methylenetetrahydrofolate reductase. Correlation with homocysteine metabolism and late-onset vascular disease. Circulation 1996;94:3074-3078.
- Schmitz C, Lindpaintner K, Verhoef P, Gaziano JM, Buring J. Genetic polymorphism of methylenetetrahydrofolate reductase and myocardial infarction. Circulation 1996;94:1812-1814.
- Boushey CJ, Beresford SAA, Omenn GS, Motulsky AG. A Quantitative Assessment of Plasma Homocysteine as a Risk Factor for Vascular Disease, JAMA 1995;274:1049-1 057.
- Allen, RH, Stabler SP, Savage DG, Lindenbaum J. Diagnosis of cobalamin deficiency I: Usefulness of serum methylmalonic acid and total homocysteine concentrations, Am J Hematol 1 990;34:90-98
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